NDV is composed of six genes that encode six structural proteins that consist of nucleoprotein (NP), phosphoprotein (P), matrix (M), fusion (F), hemagglutininneuraminidase (HN), and the RNA polymerase (L). Lentogenik cause respiratory mild or subclinical infections in young poultry, mesogenik cause respiratory and nervous while velogenik cause respiratory, digestive and nervous with a high mortality rate of over 80% (Kim et al., 2007). Category severity of illness depend on the strain of virus that is lentogenik, mesogenik and velogenik.
The clinical manifestations that arise from mild to severe. This disease can occur within a few hours to several weeks. Introduction Newcastle Disease (ND) is a viral disease of acute and highly contagious in poultry caused by avian paramyxovirus, family Paramyxoviridae namely type 1 or Newcastle disease virus (NDV). Keywords: Newcastle Disease, poultry, PCR 1. Primer pair AA-HL with a length of 362 nucleotides capable of amplifying the samples tpm3-2015, trm and KtM The conclusion showed that in the district of Malang found ND viruses that infect chickens.
Results showed that chicken from the village of Kambingan, District Tumpang (KtM1-2015), District Tumpang (tpm3-2015), and District Turen (trm6-2015), based on the test showed positive HI titers of virus in a row 8, 8 and 128. DNA amplification process is carried out using PCR Primer OneStep, using the primer pair of genes F (primer AAHL sense dan AAHL antisense). The research method is descriptive by detecting the presence of the virus through clinical symptoms, tests HI (Hemaglutination Inhibition) and the PCR test. This study aims to detect ND virus in Malang. The presence of ND virus in Malang until now has not been proven scientifically. ND virus molecular characteristics are mostly located in genes F, in the variable region that contribute to the fusion of the virus in the host. The existence of Newcastle disease in Malang is still very high considered of much circulation ND vaccine lived in the field. Keywords: wound healing, red betel, neutrophile, macrophage, fibroblast, angiogenesis, collagen density BIO-1Ģ OB-02 Detection of Newcastle Disease Virus in Chicken of the District of Malang, East Java, Indonesia Dahliatul Qosimah*, Sri Murwani, Riski Arya Pradikta, and Indah Amalia Amri Department of Microbiology and Immunology, Brawijaya University Malang, Indonesia Abstract-Disease Newcastle disease (ND) is caused by newcastle disease virus, Family Paramyxoviridae, acute and highly contagious in poultry, especially chickens that can cause the death of up to %.
Results showed that P4 group (group wound with red betel leaf s extract 12.5%) can improve the histopathology image in rat s skin infected by S. Collagen density was analyzed using Kruskall-Wallis test and Mann-Whitney test. Hypothesis test such as total of neutrophile, macrophage, fibroblast, and angiogenesis were analyzed using ANOVA and LSD. It was designed for 20 Westar male rats, in 3 month ages and divided into 5 group, P0 was negative control group wound without medical treatment, P1 was positive control group wound with povidone iodine, P2 was group wound with red betel leaf extract s 50%, P3 was group wound with red betel leaf extract 25% and P4 was group wound with red betel leaf s extract 12.,5%. Laboratory experiment used a randomized post-test design. 1 SECTION IV: BIOLOGY, PHARMACY AND AGRICULTURE OB-01 The Effect of Giving Piper crocatum Extract on the Histopathological of White Mice's Wound Infected by Staphylococcus aureus I Dewa Putu Anom Adnyana 1*, Wurlina 2, Rochiman Sasmita 2, Dewa Ketut Meles 3, and Annisa Mutmainnah 4 1 Pharmacology Veterinary, Faculty of Veterinary Medicine University of Brawijaya, Malang, Indonesia, 2 Reproduction Veterinary, Faculty of Veterinary Medicine Airlangga University, Surabaya, Indonesia 3 Pharmacology Veterinary, Faculty of Veterinary Medicine Airlangga University, Surabaya, Indonesia 4 Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia Abstract-The aim of this research was to show the effect of red betel leaf s extract (Piper crocatum) on the histopathology image in Westar rat s skin which infected by bacteria Staphylococcus aureus.